This group included 12 (30%) adults and 2 (2.2%) children. Other abnormalities in the pseudodiploid group were t(1;19) in 2 (1.5%) patients, -7/7q deletion in 3 (2.3%), 11q23 abnormality in 2 (1.5%), and 6q del in one (0.8%) (figure 2 and table 2). Other pseudodiploidy karyotype groups (except for the main abnormalities) were detected in the
second large group of cytogenetic abnormalities in 10 (7.8%) patients. These abnormalities were t(10;12), inv12, t(4;9), t(1;4), t(7;14), del X, dup 1, and del 12 (each of them in one [1.13%] patient and t(6;12) in 2 [2.27%] patients). The details of these abnormalities are provided in table 3. Figure 1 This graph illustrates the Inhibitors,research,lifescience,medical distribution of cytogenetic abnormalities in our T-cell acute lymphoblastic leukemia patients. Table 1 Distribution of the cytogenetic abnormalities Inhibitors,research,lifescience,medical in the T-cell acute lymphoblastic leukemia patients Figure 2 This graph depicts the distribution of the karyotypes of our 128 B-precursor acute lymphoblastic leukemia patients. *P value is statistically significant Table 2 Distribution of the karyotypes of 128 B-precursor acute lymphoblastic leukemia patients Table 3 Distribution of the other Inhibitors,research,lifescience,medical cytogenetic abnormalities in pseudodiploid B-precursor acute lymphoblastic
leukemia pediatric patients Discussion In this study, we present cytogenetic findings on 168 adult and pediatric ALL patients in Fars province and compare our findings Inhibitors,research,lifescience,medical with the relevant reports in the literature. We had a successful cell culture rate of 84.5%, which is comparable to those in the studies by Silva et al.8 and Pérez-Vera et al.5 who had successful cell culture rates of 91% and 87.5%, respectively. Unsuccessful cell cultures may be due to the nature of malignant cells as well as technical
and transport problems. In the present study, abnormal karyotypes were found in 61.7% of our B-ALL cases. Usvasalo et al.9 showed that using advanced methods in cytogenetics such as polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) could augment the detection of abnormal cytogenetics in leukemic patients Inhibitors,research,lifescience,medical and, the authors detected chromosomal aberration in 85% of the cases. Silva et al.8 detected abnormal cytogenetics in 92.3% of their patients. These differences are due to the use of the conventional G-banding technique versus more developed cytogenetic analytical methods. In the present study, 38.3% of our oxyclozanide B-ALL cases showed normal karyotypes, which was similar to the figure reported by the Xin Li et al.4 study (39%). There were slightly fewer normal karyotypes in our pediatric B-ALL patients (37.5%) than in our adult B-ALL patients (40%); Bcr-Abl inhibitor however, the difference was not statistically significant (P>0.05). In the B-ALL group, t(9;22) was the most frequent chromosomal translocation (11%). Moorman et al.10 reported 19% positivity of this translocation, but they performed both FISH and RT-PCR. Mancini et al.