The method gave reproducible results of assay for all the samples tested for tobramycin. The excipients in the formulation and the probable degradation products of tobramycin as selleckchem Baricitinib a result of accelerated storage did not interfere with the estimation of the component. The assay of the test samples (RT and accelerated) and innovator samples are summarized in Table 4. Table 4 Application of the developed HPLC method to the determination of tobramycin in dosage forms CONCLUSIONS A HPLC method based on UV detection has been developed and validated for determination of tobramycin from an ophthalmic solution. The method is simple, rapid, specific, accurate (error 0.80%), precise (RSD <2.0%), and linear (r2=0.9998). The described method is suitable for routine analysis and quality control of the ophthalmic solution and injection containing tobramycin.
Footnotes Source of Support: Nil Conflict of Interest: None declared.
Science and technology have never been so promising nor have delivered so many opportunities to improve health and extend lives, but continued investments are being invested in both the public and private sector, in spite of the current economic climate.[1�C3] Increasing pharmaceutical industry success rates and delivering more medicines are very challenging, but very few predictive scientific and analytical tools are available. Research on drugs involves production control of bulk drug and final product, toxicological analysis of side effects of the drug or its possible impurities, and determination of the fate of a drug and its metabolites in an organism by the monitoring of body fluids.
 Common criteria for drug evaluation include the quality and therapeutic value of the bulk drug and pharmaceutical product, identification studies, purity, content, uniformity, chemical and physical stability, and biological availability.[6,7] PHARMACEUTICAL ANALYSIS AND HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY��AN OVERVIEW Analysis of pharmaceutical compounds and newer drugs is commonly used in all the stages of drug discovery and development process. These analytical techniques provide more accurate and precised data, not only supporting drug discovery and development but also postmarket surveillance. Pharmaceutical analysts work regularly to improve the reliability of existing techniques to cope up the demands for better chemical measurements.
Modern pharmaceutical analysis is mainly dominated by costlier instrumental analysis. Hence, many analysts�� focus is on developing newer applications, Carfilzomib discoveries, and new methods of analysis to increase the specificity and sensitivity of a method.[9,10] Analytical methods used in drug analysis are diversified and are still being improved to find better solutions to satisfy manufacturers and institutions that test drug quality.