Interestingly, there was an 18% and 20% greater GH response (p > 0.05) during T3 and T4 versus T2, respectively, while a 42% difference was found between T5 and T2 (p > 0.05). Although these responses

were not significantly different, it does suggest an interesting trend that provided some support to previous results [57]. Whether the high dose glutamine ingestion played a role in this response is not clear. Previous investigations have suggested that glutamine concentrations can elevate the GH response at rest [58, 59], but not exercise [58]. It appears that the most compelling stimulus for glutamine’s role in stimulating GH release is during see more prolonged critical illness when plasma glutamine concentrations are below normal levels [60]. Thus, the high variability in the GH response in this study may be attributed to the normal glutamine concentrations at rest, however the largest gains in GH occurred during the trial (T5) that glutamine concentrations were significantly higher than T2 – T4. In conclusion, the results of this study demonstrate that AG supplementation provides significant ergogenic benefits by increasing time to exhaustion during a mild hydration stress. This ergogenic effect was Selleck Dinaciclib likely mediated by an enhanced fluid and

electrolyte uptake. AG supplementation, irrespective of dosing, did not have any effect on immune, inflammatory or oxidative stress responses. Results also indicated that the AG supplement did not influence the pituitary-adrenal-testicular axis during this exercise and mild hypohydration perturbation. Acknowledgements This study was funded by Kyowa Hakko Bio Co., Ltd. References 1. Nose H, Morimoto T, Ogura K: Distribution of water losses among fluid compartments of tissues under thermal dehydration in the rat. Jpn J Physiol 1983, 33:1019–1029.CrossRefPubMed 2. Senay LC, Pivarnik JN: Fluid shifts during exercise. Exerc Sport Sci Rev 1985, 13:335–387.CrossRefPubMed 3. Hoffman JR, Stavsky H, Falk B: The effect of water restriction on anaerobic power and vertical jumping height in basketball players. Int J Sports Med 1995, 16:214–218.CrossRefPubMed 4. Carter JE, Gisolfi

CV: Fluid replacement during and after exercise in the heat. Med Sci Sports Exerc 1989, 21:532–539.PubMed 5. Armstrong LE, Casa DJ, Roti MW, Lee EC, Craig SA, Sutherland JW, Fiala KA, Maresh CM: Influence of betaine consumption on strenuous running Miconazole and sprinting in a hot environment. J Strength Cond Res 2008, 22:851–860.CrossRefPubMed 6. Lima AA, Carvalho GH, Figueiredo AA, Gifoni AR, Soares AM, Silva EA, Guerrant RL: Effects of an alanyl-glutamine-based oral rehydration and nutrition therapy solution on electrolyte and water absorption in a rat model of secretory diarrhea induced by cholera toxin. Nutrition 2002, 18:458–462.CrossRefPubMed 7. Nath SK, Dechelotte P, Darmaun D, Gotteland M, Rongier M, Desjeux JF: ( 15 N) and ( 14 C) glutamine fluxes across rabbit ileum in experimental diarrhea.