n. Subperithecial tissue in section. o. Asci with ascospores in cotton blue/lactic acid. Scale bars: a, c, e, g = 0.5 mm. b, d, f = 0.3 mm. h = 0.1

mm. i, m–o = 10 μm. j = 25 μm. k, l = 5 μm Anamorph: Trichoderma selleck compound koningii Oudem. in Oudemans & Koning, Arch. Néerl. Sci. Exactes Nat., Sér. 2, 7: 291 (1902). Fig. 7 Fig. 7 Cultures and anamorph of Hypocrea koningii (CBS 119500). a–c. Cultures at 25°C (a. on CMD, 14 days; b. on PDA, 13 days; c. on SNA, 14 days). d. Hyphae on agar surface (SNA, 15°C, 3 days). e, f. Chlamydospores (e. intercalary, f. terminal; 11 days). g–j. Conidiation on SNA, observed in the stereo-microscope (g. pustules, 25°C, 7 days; h–j. on aerial hyphae; h, i. 25°C, 3 days, j. 15°C, 8 days). k–n. Conidiophores (k. showing lageniform and ampulliform phialides; LY294002 supplier 5–6 days). o, p38 MAPK inhibitor p. Phialides (5 days). q Conidial chains (7 days). r–u Conidia (6 days). e, f, k–u. On CMD, at 25°C. Scale bars: a–c = 15 mm. d = 50 μm. f, s, u = 5 μm. g = 3 mm. h–j, q = 30 μm. l–n = 15 μm Stromata when fresh 0.5–3 mm diam,

0.5 mm thick, solitary or gregarious, pulvinate, smooth, lively orange-brown. Stromata when dry (0.4–)0.8–1.8(–2.4) × (0.3–)0.6–1.3(–1.5) mm (n = 30), 0.15–0.45 mm (n = 20) thick; flat pulvinate, discoid or lenticular; margin free. Outline circular or oblong. Ostiolar dots (17–)22–34(–38) μm (n = 30) diam, typically invisible, only rarely distinct, convex to semiglobose, hyaline, or with a dark ring. Stromata when young white, the centre turning pale yellow or orange, eventually dark orange-brown to reddish brown, 7–8CE7–8, with or without white mycelial margin. Rehydrated stromata light orange-brown; ostiolar openings minute, hyaline; surface smooth, slightly velutinous. No change seen in 3% KOH. Stroma anatomy: Ostioles (42–)49–70(–84) mafosfamide μm long, projecting to 15 μm, (12–)17–37(–50) μm wide at apex (n = 20), conical, without conspicuous apical cells. Perithecia (130–)145–180(–195) × (93–)110–160(–175) μm (n = 20), globose or flask-shaped. Peridium (11–)13–17(–20)

μm (n = 20) thick at the base, (6–)9–14(–16) μm (n = 20) thick at the sides, hyaline. Cortical layer (13–)16–23(–27) (n = 30), an orange-brown t. angularis of minute thin-walled cells (2–)3–6(–7) μm long (n = 60) in face view and in vertical section. Hairs on mature stroma (6–)8–11(–12) × 2–4(–6) μm (n = 20), 1–2 celled, ends rounded, cylindrical or globose, smooth or warty, yellow-orange to pale brown, surface warty by projecting cells. Subcortical tissue a loose t. intricata of hyaline thin-walled hyphae 2.5–4.0(–4.5) μm (n = 10) wide. Subperithecial tissue a dense t. epidermoidea of hyaline thin-walled cells (5–)6–14(–20) × (3–)4–9(–13) μm (n = 30). Stroma sides of a thin layer of narrow hyphae (2.0–)2.5–4.5(–5.0) μm (n = 10) wide.