selleck inhibitor However, there were no significant differences in water contact angles between the two groups except for Selleckchem AZD0156 Ti-6Al-4 V. Of the various materials, the surface of Co-Cr-Mo demonstrated the highest water contact angle in both groups. The results of the adhesion of S. epidermidis to both groups of the various specimens are shown in Figure 2. Larger amounts of S. epidermidis adhered to each specimen in the coarse group than in the fine group. In particular, Oxinium, Ti-6Al-4 V and SUS316L demonstrated statistically significant differences between the fine group and the coarse group (P < 0.05). The Co-Cr-Mo specimens LY2835219 in the fine group had significantly lower adherence than the Ti-6Al-4 V, Cp-Ti and SUS316L specimens (P < 0.05). Similarly, the Co-Cr-Mo specimens in the coarse group exhibited significantly lower amounts of adhered bacteria than the other four materials (P < 0.05). Figure 1 SEM micrographs. The fine group specimens had a relatively featureless surface compared to the coarse group specimens. Fine group: Oxinium (a), Co-Cr-Mo (b), Ti-6Al-4 V (c), Cp-Ti (d), SUS316L (e). Coarse group: Oxinium (f), Co-Cr-Mo (g), Ti-6Al-4 V (h), Cp-Ti (i), SUS316L
(j). Original magnification × 5000 (Scale bar =1 μm). Table 1 Surface roughness Ra (nm) Fine group Coarse group P-value Oxinium 8.5 (0.5)b,d,e 30.0 (2.0)b,e 0.004 Co-Cr-Mo 5.8 (0.2)a,c,e 12.0 (1.9)a 0.004 Ti-6Al-4 V 7.1 (0.4)b,d,e 16.5 (14.5) 0.003 Cp-Ti 5.6 (1.2)a,c,e 22.0 (6.0) 0.004 SUS316L 1.8 (0.4)a,b,c,d 7.2 (1.9)a 0.002 Data were expressed as a mean (standard deviation (SD)). Ra: arithmetic mean of the departure of the roughness profile from the profile center-line. a P < 0.01 compared with Oxinium. b P < 0.01 compared with Co-Cr-Mo. c P < 0.01 compared with Ti-6Al-4 V. d P < 0.01 compared with Cp-Ti. e P < 0.01 compared with SUS316L. Table 2 Contact angles of deionized water (degree) Contact angle (degree) Fine group Coarse group
about P-value Oxinium 73.9 (5.6)b,d,e 76.3(9.2) b,c,d,e 0.33 Co-Cr-Mo 104.1 (5.7)a,c,d,e 105.8 (1.0) a,c,d,e 0.06 Ti-6Al-4 V 77.0 (5.3)b,d,e 84.7 (3.0) a,b,e 0.002 Cp-Ti 89.2 (7.1)a,b,c 84.8 (3.0) a,b 0.20 SUS316L 90.0 (2.3) a,b,c 91.2 (2.0) a,b,c 0.39 Data were expressed as a mean (standard deviation (SD)). A greater water contact angle means a more hydrophobic surface. Oxinium had the smallest water contact angle, indicating the most hydrophilic surface. a P < 0.01 compared with Oxinium. b P < 0.01 compared with Co-Cr-Mo.