5, 5, 10, 15, 30, 45, 60 min, after which, 0.05 pmol 5′-end fluorescein-labelled oligonucleotide (dT)35 was added. The samples were then loaded onto 2% agarose gels without ethidium bromide ZD1839 purchase and separated by electrophoresis in a TAE buffer as described for EMSA tests. The incubation periods for each temperature, where 50% of (dT)35 was bound, were noted. Protein sequence analysis The amino acid sequences of studied SSB proteins were analyzed using standard protein–protein BLAST and RPS-BLAST. Multiple sequence alignment was generated in ClustalX, using a PAM 500 scoring matrix. The results were prepared using the GeneDoc editor program (http://​www.​psc.​edu/​biomed/​genedoc).

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