��100%??(1)dy?[23]:Frequency??of??2n??pollen??grains=2��dy+tri2��dy+3��tri+4��tet, http://www.selleckchem.com/products/Imatinib-Mesylate.html = total number of dyads observed; tri = total number of triads observed; tet = total number of tetrads observed.2.4. PhotomicrographsPhotomicrographs from the freshly prepared desirable slides having clear chromosome counts, dyads, triads, tetrads, and pollen grains were taken with a digital imaging system of Leica QWin. 3. ResultsMeiosis in one of the accession collected from Bharmour, 2,300m was totally normal with the presence of 14 bivalents at diakinesis (Figure 2(a)) and regular 14:14 segregation of chromosomes at opposite poles (Figures 2(a) and 2(c)) leading to normal tetrads with four n microspores (Figure 2(d)) and consequently n pollen grains (21.98�C26.35��m �� 20.82�C24.01��m, Figure 2(e)) and 100% pollen fertility.

However, the second accession also collected from Bharmour, 2,300m showed highly abnormal meiosis characterized by the erratic behaviour of chromosomes at different stages of meiosis I and II.Figure 2(a�Ce) Meiocytes with normal meiotic behaviour in R. laetus. (a) A PMC with 14 bivalents at diakinesis. (b) A PMC showing 14:14 chromosomes distributions at A-I. (c) A PMC showing two poles at A-I. (d) A tetrad with four n (reduced) …3.1. Chromosomal Behaviour during Meiosis IAnalysis of PMCs at M-I of meiosis I revealed that all the PMCs showed the presence of 28 chromosomes as univalents which either remained randomly dispersed in the cytoplasm or shifted towards the periphery or in the centre or in 2�C5 groups in the PMCs (Figures 3(a)�C3(c)).

Furthermore, the movements of chromosomes at A-I is very irregular, and in most of the PMCs they lagged behind (57.83%, Figure 3(d)). In the majority of the cases these lagging chromosomes did not get included into the telophase nuclei and formed micronuclei at T-I (Figure 3(e)). In some of the PMCs, segregation of chromosomes at A-I was irregular and the most common distribution was observed to be 11:17 (Figure 3(f)). In many PMCs it was also noticed that chromosome failed to move towards the A-I poles and remained in the centre of the PMC to form restitution nuclei (Figures 3(g) and 3(h)). Even some of the PMCs showed thick chromatin bridges at anaphases and telophases which did not allow the separation of chromatin material and thus formed restitution nuclei (Figure 3(i)).Figure 3(a�Ci) Meiocytes with erratic male meiosis at first meiotic division in R. laetus. (a) A PMC with 28 randomly dispersed univalent chromosomes at M-I. (b) 28 univalent chromosomes positioned towards the periphery AV-951 of PMC at M-I. (c) In one of the …3.2.