Thus, considering the number of introns reported here, B. emersonii’s gene structure appears to be more similar to that observed in ascomycetes. Further evidence suggesting that B. emersonii gene structure is more similar to ascomycetes is the average intron length observed in this aquatic fungus. We detected introns ranging from 55 Ganetespib clinical trial to 333 nucleotides, an intron length more similar to that observed in the ascomycete species [49–51]. However, it is relevant to notice that even fungi belonging to the same class

present different gene structures, as the case of Ustilago maydis, a basidiomycete that possesses an average number of introns per gene smaller than one [52, 53]. To further characterize the intron structure of B. emersonii genes, we have identified the splicing junctions present in the introns sequenced from iESTs. AZD0156 manufacturer We observed that most of the introns showed the canonical splicing sites and the consensus branch site sequence similar to those detected in introns from genes previously characterized in B. emersonii. These observations suggest that inhibition of splicing by stress in B. emersonii is probably a random process opposite to a selective inhibition of some specific pre-mRNAs based on different intron-recognition sequences. The fact that B. emersonii possesses proteins involved

in pre-mRNA processing containing zinc-related domains indicates that one

possible mechanism by which cadmium inhibits splicing in this fungus could be the Ribociclib displacement of zinc ions from these proteins. This hypothesis is consistent with the fact that we did not observe a global repression in the transcription of genes encoding spliceosome proteins under these stress conditions. Additionally, the hsp70-1 gene intron was not found to be retained when B. emersonii cells were treated with hydrogen peroxide. These data suggest that splicing blockage is not due to an indirect effect of oxidative stress caused by cadmium. Furthermore, Shomron and collaborators [54] demonstrated that zinc is an essential factor for the second step of the splicing reaction, suggesting that putative zinc-dependent MM-102 price metalloproteins are required for this step of RNA splicing process. Interestingly, a recent report demonstrated that cadmium, a metal that presents many chemical similarities to zinc, in low quantities can restore in vitro mRNA splicing inhibited by zinc-depletion [55]. These results indicated that cadmium could effectively substitute zinc in metalloproteins, including those present in the spliceosome machinery [55]. Nevertheless, at higher concentrations the authors observed that cadmium caused the opposite effect, inhibiting splicing in vitro [55].