The marker Mho-53 was the most Compound C mouse discriminatory VNTR, displaying six different allele sizes with repeat copy numbers ranging from 3 to 8, depending on the isolate. The marker Mho-116 was the most homogenous marker, as almost all of the isolates harboured one repeat (three harboured two copies). This finding click here was reflected by the diversity index of each VNTR, estimated GW4869 nmr from the HGDI, with a value of 0.784 for the most discriminatory marker (Mho-53) and a value of 0.020 for the less discriminatory one (Mho-116). The overall discriminatory index of the MLVA assay was 0.924. Table 2 Number of repeat units for the five VNTR markers MLVA type No. of repeats at the following VNTR loci
Mho-50 Mho-52 Mho-53 Mho-114 Mho-116 1 1 8 8 1 1 2 1 8 3 1 1 3 1 8 3 2 1 4 1 8 4 1 1 5 1 8 4 2 1 6 1 8 4 2 2 7 1 8 5 1 1 8 1 8 5 2 1 9 1 8 6 1 1 10 1 8 6 2 1 11 1 8 7 1 1 12 1 8 7 2 1 13 1 8 8 2 1 14 3 8 3 1 1 15 1 9 3 2 1 16 1 9 4 1 1 17 1 9 4 2 1 18 1 9 5 2 1 19 2 8 3 1 1 20 2 8 3 2 1 21 2 8 4 1 1 22 2 8 4 2 1 23 2 8 5 2 1 24 2 9 7 1 1 25 3 8 3 2 1 26 3 8 4 1 1 27 3 8 4 2 1 28 3 8 5 2 1 29 3 8 6 2 1 30 3 8 7 2 1 31 3 9 4 2 1 32 3 9 7 2 1 33 4 8 3 2 2 34 4 8 4 2 1 35 4 8 5 2 1 36 4 8 6 2 1 37 5 8 4 2 1 38 1 10 3 2 1 39 1 10 4 2 1 40 1 10 5 2 1 A combined analysis of
the five VNTR loci in the 210 M. hominis isolates revealed 40 MLVA types (Table 2). Three MLVA types, 5, 8 and 10, were present in more than 20 isolates. In 18 cases, one unique MLVA type was observed in a single patient. Interestingly, the two ATCC strains, H34 and M132, had the identical MLVA type 10, while the PG21 ATCC strain belonged to the MLVA type 36. The 167 urogenital isolates were classified into 34 MLVA types (Additional file 1: Phospholipase D1 Table S1). The 34 extragenital isolates contained 14 MLVA types, including eight MLVA types that had already been described for urogenital isolates. One set of two concomitant extragenital isolates (Mh-2537, Mh-2539) collected from the same patient was analysed. The MLVA typing led to an identical MLVA profile for both isolates (Additional file 1: Table S1). Sequential isolates (obtained on time intervals from one week to six months) from each of seven patients revealed no intra-individual variation in six of them, suggesting that they suffered from a persistent M. hominis infection.