The cells were surface stained with anti-CD3+ and anti-CD4+ antibodies or anti-CD3+ and anti-CD8+ and analyzed by flow cytometry (P < 0.001). Immunofluorescence analysis and histological changes in the livers of recipient mice Immunofluoresence analysis of the liver sections of the transgenic mice showed infiltration of high number of the CFSE labeled cells, when they received transfer from immunized mice (Figure 9a). H&E staining of the liver sections for the same group of recipient mice showed infiltration of lymphocytes beside

the histological changes, selleck chemicals llc such as steatosis, due to the expression of transgenes (Figure 9b). Interestingly, the infiltrated cells were concentrated in the areas where there was steatosis. On the other hand, the transgenic mice receiving cells from Saracatinib clinical trial non-immunized donors showed few CFSE labeled cells on the liver sections and no cell infiltration was observed in the H&E stained liver section (Figure 10a, b). The non-transgenic mice showed no histological changes and no infiltration of CFSE labeled cells, whether they received donor cells from immunized (Figure 9c,

d) or non-immunized mice (Figure 10c, d). Thus, repetitive transfer of splenocytes from HCV immunized mice into HCV transgenic mice may be needed in order to increase inflammation in the liver. Figure 9 Histological alterations in livers selleck from transgenic and non-transgenic mice injected with CFSE-labeled splenocytes from immunized mice. A) Immunofluorescent analysis of frozen liver sections (5 μm thick) of a transgenic mouse showing CFSE labeled cells scattered over all the liver section. The fluorescent cells are indicated by arrows. B) H&E stained liver section of transgenic mouse showing steatosis. There is infiltration

of lymphocytes in the liver which is concentrated close to hepatic steatosis (indicated by arrows). C) Immunofluorescence analysis of frozen liver sections (5 μm thick) of non-transgenic mouse showing no GBA3 CFSE labeled cells over the liver section. D) H&E staining of liver section of non-transgenic mouse showing normal histology of the liver and no lymphocyte infiltration. Scale bar = 50 μm. Figure 10 Histological alterations in livers from transgenic and non-transgenic mice injected with CFSE-labeled splenocytes from non-immunized mice. A) Immunofluorescent analysis of frozen liver sections (5 μm thick) of a transgenic mouse showing few CFSE labeled cells scattered over all the liver section. B) H&E stained liver section of transgenic mouse showing steatosis. There is no infiltration of lymphocytes in the liver. C) Immunofluorescence analysis of frozen liver sections (5 μm thick) of non-transgenic mouse showing no CFSE labeled cells over the liver section. D) H&E staining of liver sections of non-transgenic mouse showing normal histology of the liver and no lymphocyte infiltration. Scale bar = 50 μm.