The aim of this

study was to evaluate the prevalence and predictors of GERD and the effect of GERD on quality of life (QOL) and pregnancy outcomes in Korean pregnant women. Methods: This study was a prospective, cohort study which followed pregnant women in the second or third trimester. Ninety-four consecutive pregnant women who visited Seoul National University Boramae Hospital for the prenatal test were included in this study. GERD was diagnosed with the use of the GERDQ (gastroesophageal reflux disease questionnaire). QOL in pregnant women with GERD was assessed using QOLRAD (quality of life in reflux and dyspepsia questionnaire). Pregnancy outcome was evaluated with obstetric records after delivery. Results: Twenty eight (29.8%) of 94 women were diagnosed as GERD by GERDQ. History of PXD101 clinical trial GERD in pre-pregnancy and BMI of pre-pregnancy were associated with the development of GERD during pregnancy (9% vs 25%, P = 0.041/ 21.04 ± 2.82 vs 19.97 ± 1.90, P = 0.036). On aspects of QOL, emotional stress (P = 0.014), sleep problem (P = 0.015), food/drink problem (P = 0.004), and vitality (P = 0.029) were more prevalent in pregnant women with GERD.

Pregnancy outcomes as assessed by birth weight, Apgar score, pre-term birth, and gestational age at partum were not different between the two groups. Conclusion: The prevalence of GERD during pregnancy was 29.8% in our cohort. Previous Selleckchem Staurosporine history of GERD and lower BMI in pre-pregnancy can be the predictive factors of the development of GERD in pregnant women. GERD significantly impaired QOL

of pregnant women. Key Word(s): 1. Gastroesophageal reflux disease; 2. pregnancy; 3. outcome; 4. Qol; 5. predictors Presenting Author: TAKAHITO KATANO Additional Authors: TSUTOMU MIZOSHITA, TAKASHI JOH Corresponding Author: TAKAHITO KATANO Affiliations: Nagoya City University Graduate School, Nagoya City University Graduate School Objective: Stem cells are generally influenced by a microenvironment niche. In the stomach, mechanism of epithelial-mesenchymal interaction has not yet fully elucidated. The aim was to produce medchemexpress a culture system to enable study of gastric epithelial-mesenchymal interaction. Methods: Glandular stomach cells from postnatal day 2 C57 BL/6 J mice were cultured in our three-dimensional (3D) primary culture system. We established mouse gastric mesenchymal myofibroblast (GMF) cell line and cocultured in collagen gels in our 3D culture system. This culture system maintains the cultured cells that are embedded in a collagen gel under an air-liquid interface environment. Results: Cultured stomach cells showed outer spindle cells and yielded expanding sphere structures, which grew for three months. In coculture system with GMF cells, the size and the number of cultured spheres were significantly greater than in control culture. The wall of cultured gastric spheres consisted of a monolayer of tall columnar cells with round nuclei at the base and mucus cytoplasm.