** See Mansfield
et al. [40] for details of the scoring system used. *** NA, not applicable. Further evidence that strains 33560 and D0121 were selleck chemicals llc unable to persistently colonize the mice is provided by the fact that while all five of the colonizing strains evoked circulating IgG2b antibody responses, the two non-colonizing strains evoked little or no antibody as shown in Figure 3. IgG2b accounts for the bulk of the antibody response of C57BL/6 IL-10-/- mice to C. jejuni [40]. Figure 3 Plasma levels of anti- C. jejuni IgG2b produced by C57BL/6 IL-10 -/- mice (first passage, experiment 2). Strains were non-adapted; each bar represents the average of five mice; whiskers indicate standard error. TSB, sham inoculated control mice. All five colonizing strains were able to cause some gross pathological changes observed at necropsy, including enlarged ileocecocolic lymph nodes, thickened colon wall, and bloody contents in the intestinal lumen (Table 3). The most common gross pathological change was the occurrence of enlarged ileocecocolic lymph nodes. In
previous experiments, in about one-third of selleck compound C57BL/6 IL-10-/- mice infected with non-adapted C. jejuni 11168, the only gross pathological change observed was an enlarged ileocecocolic lymph node and the histopathology score at the ileocecocolic junction was ≤ 10 (Grade 0). Four of the five colonizing strains were able to produce histopathological changes at the ileocecocolic junction that resulted in a histopathology score ≥ 10 in at least one mouse in the initial passage (Table 3). (See [40] for details of the scoring system used. Briefly, the intestinal lumen and three layers of the intestinal Astemizole wall (mucosa, lamina propria, and submucosa) were evaluated separately for indicators of inflammation such as excess mucus, tissue hyperplasia,
tissue architecture and integrity, infiltration of monocytes and neutrophils, edema, fibrosis, and vasculitis. Characters contributed to a score that ranged from 0 to 44; scores less than 10 were considered normal.) Three C. jejuni strains caused more Sotrastaurin severe enteritis following serial passage (experiment 2, serial passage experiment) For colonizing C. jejuni strains, the initial results described above were obtained in the first of four serial passages. For subsequent passages, C. jejuni growth from cecal tissue of each individual mouse was harvested and used as the inoculum for the next serial passage. All of the C.