Research on F. solani–sea turtle interactions has gained increasing interest because this fungus has being isolated from dead eggs in natural nests of several different sea turtle species GSK1120212 mouse at different locations (Phillott & Parmenter, 2001; Phillott et al., 2001;

Marco et al., 2006; Abella et al., 2008). Identification of potential pathogens threatening endangered sea turtle species (IUCN, 2009) is crucial for the development of conservation plans. In this study, we have morphologically and molecularly characterized 25 isolates of F. solani associated with egg mass mortalities of loggerhead sea turtle, Caretta caretta, on Boavista Island. This island represents one of the most important nesting regions for this species. The hatching success of this species is currently severely threatened as a high number of their nests contained eggs with symptoms of fungal infection. This has resulted in a high hatching failure rate. Loggerhead sea turtle eggs showing symptoms of fungal infection were collected from sea turtle nests located in Ervatao, selleck chemicals Joao Barrosa and Curral Velho beaches on Boavista Island (Cape Verde, Africa). The fungus was isolated from internal and external symptomatic areas of egg shells that exhibited unusual colored spots (yellow,

blue, grayish) compared with healthy ones, from eggs shells with severe symptoms of infection characterized by grayish mycelium covering the shell (Fig. 1a–c), and also from infected embryos (Fig. 1d). For isolations, a glass-ring technique was used according to the methodology of Cerenius et al. (1987), and pure cultures were maintained on peptone glucose agar (PGA) (Söderhäll et al., 1978) with penicillin (100 mg L−1). Cultures were labeled as 001AFUS through 058FUS in the culture collection of the Real Jardín Botánico (Madrid, Spain) (see Table 1). Fungal spores and mycelia were examined microscopically under an Olympus BX-51 compound microscope (Olympus Baricitinib Optical, Tokyo, Japan) and species characterization was

performed following the manuals for Fusarium spp. identification of Booth (1977) and Nelson et al. (1983). Light micrographs were captured using a Micropublisher 5.0 digital camera (Qimaging, Burnaby, BC, Canada) and the software syncroscopy-automontage (Microbiology International Inc., Frederick, MD) as described in Diéguez-Uribeondo et al. (2003). For molecular characterization, DNA extraction was carried out by growing the mycelium as drop cultures (Cerenius & Söderhäll, 1985). Genomic DNA was extracted from these cultures using an E.Z.N.A-Fungi DNA miniprep kit (Omega Biotek, Doraville, GA). DNA fragments containing internal transcribed spacers ITS1 and ITS2 including 5.8S were amplified and sequenced with primer pair ITS5/ITS4 (White et al., 1990) as described in Martín et al. (2004). Nucleotide blastn searches with option standard nucleotide blast of blastn 2.