Our studies show that elevated Aurora A term, a standard oncogenic function in human cancers, GW0742 has the dominant negative effect of inactivating p73 purpose through increased phosphorylation of the protein sequestered in the cytoplasm. The positive correlation between Aurora A overexpression and cytoplasmic p73 localization in human pancreatic cancer tissue corroborate the experimental findings and shows that these tumors have weakened or inactivated DNA and spindle destruction induced apoptosis and SAC trails, making them refractory to main-stream radiation and chemotherapeutic regimens. Step by step studies of p73 phosphorylation pages of these tumors as well as chemosensitivities and radiosensitivities could help resolve the issue and future design of accordingly targeted therapies. In conclusion, we discovered a pathway of Aurora Ap73 axis where Aurora p73 function is inactivated by A phosphorylation in both DNA damage induced cell death and mitotic SAC trails. More comprehensive studies of Aurora A involvement in both signaling pathways will enhance our understanding of oncogenic function of Aurora A in cancer biology and help us Organism create more efficient approaches for cancer prevention and treatment. All cell lines were obtained from ATCC. Immunohistochemical staining for Aurora A, p73, and p53 was done on 4 mm unstained sections from tissue microarray blocks consisting of 114 PDAC and 20 pancreatic tumefaction cells from patients who’d withstood pancreaticoduodenectomy at M. D. Anderson and UAB, respectively. The studies were approved by both institutional review boards. Detailed experimental procedures are available in the Supplemental Experimental Procedures. For transfection, Fugene 6 transfection reagent, oligofectamine, and lipofectamine 2,000 were used in accordance with manufacturers guidelines. For luciferase assays, H1299 or Saos 2 cells were cotransfected with exactly the same amount of CAL-101 PI3K inhibitor WT or mutant pEGFPp73a, luciferase reporter construct, and central control Renilla luciferase expression plasmid, with or without increasing amounts of Flag Aurora A WT or KD expression plasmids. The total amount of plasmid DNA was kept constant at pcDNA3. We tested luciferase activities 24 hr after transfection employing a double luciferase reporter assay kit. Additional siRNAs for both genes from Santa Cruz Biotechnology were also used. Biochemical protein fractionation of cells was performed according to the manufacturers protocol. Whole cell extracts were prepared in RIPA buffer. All other experimental methods, conditions, and primer sequences for semiquantitative RT PCR have already been described. p73 proteins, created by an in vitro transcription and translation set, were incubated with 32P labeled p21 probe containing the p53 DNA binding site in the binding buffer at room temperature for 20 min. For the competition analysis, 1 mg of unlabeled probe was added to the effect.