Methods: The fruit extract of Limonia acidissima Linn was obtained by macerating 3 times with methanol and then concentrating it at reduced pressure. Bioassay of the extract was carried out to assess the anticancer activity. Breast cancer cell lines, SKBR3 and MDA MB435, were used for in-vitro cell proliferation, cell viability assay, and cell CFTRinh-172 inhibitor cycle analysis of the extract.
Results: Bioassay of the extract of Limonia acidissima Linn. showed that a fraction (fraction 3) of the ethanol extract had anticancer
activity against SKBR3 and MDA-MB435 human breast cancer cells. The effective dose (ED50) of Limonia acidissima Linn. fraction 3 was 56.1 and 30.6 mu g/ml for SKBR3 and MDA-MB435, respectively. After 48 h of exposure, this fraction (100 mu g/ml) significantly reduced IPI145 cell proliferation in both cancer cell lines. In MDA-MB435 cells, cell cycle analysis showed that fraction 3 induced the accumulation of cells in G2/M phase, but no significant change in cell cycle
was detected in SKBR3 cells.
Conclusion: The results indicate that the extract fraction could induce cell cycle arrest. However, further investigation is needed to assess the molecular mechanisms of the anticancer activities of this plant.”
“A rare case is presented concerning retroperitoneal hemorrhage in a 28-year-old patient after a cold knife conization and cervical suture reconstruction. Arteriovenous cervicoisthmic malformation is a risk factor that was identified see more in a hysterectomy sample.”
“Introduction: Pediatric patients supported by ventricular assist devices (VADs) are becoming more common. No guidelines exist to provide
an approach to diagnosis or management of circulatory arrest in these patients. We present two case reports of apparent circulatory arrest in pediatric VAD-supported patients at our institution.
Discussion: Diagnosis of circulatory arrest in VAD-supported patients can be challenging, especially with non-pulsatile flow devices. Traditional methods of diagnosis, such as pulse check and blood pressure, may be unreliable. Etiology may be primary device failure though other etiologies need to be considered. Treatment of the circulatory arrest depends on the etiology and type of VAD device being used.
Conclusion: In the absence of strong literature on the approach to these patients, we present these case reports and our local institutional guidelines as a starting point for discussion. (C) 2012 Elsevier Ireland Ltd. All rights reserved.”
“Purpose: To investigate the immunomodulatory and antitumor activities of ligustilide (LIG) extracted from Angelica sinensis in mice.
Methods: Normal and tumor-bearing Institute of Cancer Research (ICR) mice were treated p. o. with LIG (5, 20 and 80 mg/kg/day) for 7 days.