Knockdown of NSBP1 resulted in a 22.6% decrease in the growth rate of cells compared with the PscNC lentivirus control cells at 96 h, decreased tumour growth in nude mice, and the induction of G(2)/M cell cycle arrest (8.78%) and apoptosis (2.19-fold). Consistent with the cell cycle arrest and apoptosis, the mRNA and protein expression levels of

cyclin B1 and Bcl-2 were decreased. In conclusion, knockdown of NSBP1 causes a statistically significant inhibition of the in vitro and in vivo growth of the PCa cell line DU145. Growth suppression is at least partially due to NSBP1 knockdown-induced G(2)/M cell cycle arrest and apoptosis. The present data provide the evidence that the NSBP1 knockdown-induced G(2)/M phase arrest and apoptosis may result from negative regulation of cyclin B1 and Bcl-2 by NSBP1, with the Smoothened Agonist resulting reduced expression of these proteins.”
“Nanostructured

Co with large lattice extension and contraction was produced by electrodepositing Co on nanoporous Au. The Co deposited showed a low magnetic saturation of 76 emu/g and a high coercivity of 462 Oe. First-principles calculations selleck chemicals showed that the magnetic moment of a Co atom is significantly decreased by lattice contraction. Therefore, the noteworthy magnetic properties of the Co deposited are attributed to the large lattice strain. Also, molecular dynamics simulation showed that the lattice extension and contraction of about 10% are generated in the overall

Co crystal. This is in agreement with the experimental results of HRTEM observation. The constraint of the movement of Co atoms by the concave structure of nanoporous Au leads to a wide spread of large strain buy GDC-0994 region. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3575327]“
“Melamine cyanurate (MCA) was utilized as an environmental friendly additive to prepare the nonhalogen flame retardant MCA/Nylon 66 composites by melt blending technique. Because of the strong hydrogen bond interactions and fine interfacial compatibility between MCA and Nylon 66, the resultant even dispersion of MCA filler in polymer matrix leads to the better toughness and strength of MCA/Nylon 66 composites than those of neat Nylon 66. Both Nylon 66 and MCA/Nylon 66 composites exhibit similar alpha-crystalline structure, but the presence of MCA influences the distribution of alpha 1 and alpha 2 crystalline phases in Nylon 66 by inducing its hydrogen-bonded sheet separation. Moreover, the blending of MCA and Nylon 66 increases the crystallization temperature and exothermicity but decreases the thermal stability of Nylon 66 and accelerates the degradation of MCA. The MCA/Nylon 66 composites show better flame retardancy at intermediate MCA contents of 10 and 15 wt %. (C) 2011 Wiley Periodicals, Inc.