Intracellular

bacterial loads were quantified at 2 and 8 h post infection by plate counting. (B) Cytotoxicity of B. pseudomallei KHW and mutants against RAW264.7 cells. Cells were infected at an MOI of 100:1. Cytotoxicity was quantified at 8 h post infection by LDH release assay. *p < 0.05. Figure S3. Secretion and function of BsaN controlled proteins. A. Secretion of BPSS1513 in strain KHW. Proteins were separated on 12% polyacrylamide gels, transferred to PVDF membranes and probed with a mouse monoclonal antibody to HA or rabbit polyclonal antibody to BopE. P: pellet; S: supernatant. B. Intracellular replication of B. pseudomallei KHW and Δ(BPSS1513-folE) mutant in RAW264.7 cells at 2 h and 8 h (MOI of 10:1) or C. 2 h and 24 h after infection at an MOI of 0.1:1. Intracellular bacterial loads were quantified by plate counting. D. Cytotoxicity of B. pseudomallei KHW and Δ(BPSS1513-folE) mutant against RAW264.7 CHIR-99021 mouse cells. Cells were infected at an MOI of 100:1. Cytotoxicity was quantified at 8 h post infection by LDH release assay. E. MNGC formation of cells infected with B. pseudomallei wild-type (WT) strain KHW and F. Δ(BPSS1513-1514) mutant at an MOI of 10:1. References 1. Galyov EE, Brett

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