Figure 8 RGD-GNR-MWNT nanoprobes for in vitro cell targeted imaging. (a) MGC803 cell selleck screening library imaged under bright-field microscopy. (b) MGC803 cell imaged under dark-field microscopy. (c) GES-1 cell imaged under bright-field microscopy. (d) GES-1 cell imaged under dark-field microscopy. RGD-GNR-MWNT nanoprobes for in vivo photoacoustic imaging Multispectral optoacoustic tomography (MSOT) is a rapidly
emerging, noninvasive, and high-resolution photoacoustic imaging system selleck compound which can achieve an isotropic and homogeneous spatial resolution of 200 μm. A near-infrared pulse laser serving as the excitation source receives PA signals for three-dimensional (3D) image reconstruction [30, 52]. RGD-conjugated sGNR/MWNT nanoprobes were applied to photoacoustic imaging to detect gastric cancer cells in in vivo subcutaneous gastric cancer xenograft model. As shown in Figure 9a, as the concentration of prepared nanoprobes increased, PA signal amplitudes also increased correspondingly. As shown in Figure 9b, compared with GNRs,
RGD-sGNR/MWNT composites could markedly enhance the MWNT PA signals at about 20%, which highly suggests that sGNRs could enhance the PA imaging Luminespib ic50 signal of MWNTs. Figure 9 Relationship curves. (a) Relationship curve between nanoprobe concentration and PA signal intensity. (b) Gold nanorod-enhanced MWNT PA signal amplitude curve at different wavelengths (black, sGNRs; red, RGD-sGNR/MWNTs). As shown in Figure 10a,b,c,d, as the post-injection time increased, the prepared nanoprobes could target actively vessels of in vivo gastric cancer tissues and accumulated more and more in the site of gastric cancer tissues. The photoacoustic signals of tumor vessels became stronger, and photoacoustic amplitudes reach the maximum at the 850-nm wavelength. Figure 10e,f showed prepared nanoprobes located inside the MGC803 cells. Our results
fully demonstrate RAS p21 protein activator 1 that RGD-conjugated sGNRs/MWNTs may be a good contrast agent for photoacoustic imaging of in vivo gastric cancer cells, and gold nanorods can enhance the PA signal of MWNTs. Golden single-walled carbon nanotubes have been used for PA imaging of in vivo tumors [30, 33]. Compared with available data, gold nanorod-modified multiwalled carbon nanotubes exhibited enhanced PA signals. Gold nanorods may have minor advantages over thin gold nanolayer for enhanced PA signals of carbon nanotubes. Figure 10 The prepared nanoprobes for photoacoustic imaging of in vivo gastric cancer cells. Photoacoustic images at (a) 1 h, (b) 3 h, (c) 6 h, and (d) 12 h post-injection. (e, f) TEM pictures of prepared nanoprobes located inside MGC803 cells.