c-FLIP over expression in stromal cells stimulated the growth and invasion of prostate cancer, including LNCaP and PC3 cells in vitro.
Conclusions: These results indicate the over expression of stromal c-FLIP and its function for promoting prostate
cancer growth and invasion.”
“The purpose of this study is to reveal characteristics of (64)Cu-labeled diacetyl-bis(N(4)-methylthiosemicarbazone) ([(64)Cu]Cu-ATSM) during cell proliferation and hypoxia by autoradiography imaging and immunohistochemical staining.
Methods: The intratumoral distribution of [(64)Cu]Cu-ATSM and [(18)F]-2-fluoro-2-deoxy-D-gloucose ([(18)F]FDG) in mice implanted with Lewis lung carcinoma (LLC1) tumor cells according to dual autoradiography were compared with the immunohistochemica staining patterns of proliferating markers [Ki-67 and 5-bromo-2'-deoxyuridine Ruxolitinib (BrdU)] and a hypoxic marker (pimonidazole). A clonogenic assay was performed using the cells of LLC1 tumor-implanted mice, and it was compared with the distribution of [(64)CL]Cu-ATSM.
Results:
[(64)Cu]Cu-ATSM mainly accumulated at the edge of rumors, whereas [(18)F]FDG was distributed inside the tumor and inside the [(64)Cu] Cu-ATSM accumulation. The number of Ki-67-positive cells/area tended to increase with [(18)F]FDG accumulation and decrease with [(64)Cu] Cu-ATSM accumulation. Selleck JNK-IN-8 On the other hand, the number of BrdU-positive cells/area was negatively correlated with [(18)F]FDG With [(64)Cu]Cu-ATSM accumulation. High [(64)Cu]Cu-ATSM accumulation was found outside the high-[(18)F]FDG-accumulation and pimonidazole-positive regions. Colony formation ability was significantly higher in the tumor cells obtained from high-[(64)Cu]Cu-ATSM-accumulation regions than the cells from the intermediate- and the
low-accumulation regions.
Conclusion: [(64)Cu]Cu-ATSM accumulation regions in tumor cells indicate quiescent but clonogenic tumor cells under mild hypoxia. [(64)Cu] Cu-ATSM could play an important role in planning appropriate tumor radiotherapy. (C) 2009 Elsevier Inc. All rights reserved.”
“Purpose: Microdialysis is a technique for monitoring the concentration of molecules in the interstitial fluid of living tissue. We these report the effects of ischemia on human renal interstitial fluid molecules.
Materials and Methods: Ten patients with a renal mass or upper tract transitional cell carcinoma who elected laparoscopic nephrectomy or nephroureterectomy were studied with in situ renal microdialysis. Microdialysate was continuously collected into separate vials every 10 minutes before and after the renal artery was stapled. Samples, were analyzed for the glucose, pyruvate, lactate and glycerol concentration.
Results: The concentration of all 4 molecules was stable throughout the pre-ischemia baseline period. Glucose and pyruvate concentrations decreased to almost zero during the first 60 minutes of ischemia.