All subjects had EUS guided biopsy with a 22G Procore needle and cell-block preparation was performed. Sections see more of cell-block material were assessed for S100A2 and S100A4 protein expression using immunohistochemistry. Results: Pre-operative biomarker assessments from EUS acquired specimens were possible in 90% (72/79) of patients, of which 14 proceeded to have pancreatectomy. Thirty-five (49%) of patients expressed S100A2 and S100A4, which were co-expressed in 97% of cases. Patients with S100A2/A4 tumours on EUS had a significantly shorter median survival (10.0 vs. 17.5

months, P = 0.03). Amongst patients with S100A2/A4 expressing tumors, pancreatectomy (n = 8) did not lead to a survival benefit Selleckchem Staurosporine compared with those with non-surgical management (n = 27) (12.5

vs. 10.0 months, P = 0.70). Of patients who had pancreatectomy, patients with S100A2/A4 expressing tumors (n = 8) had shorter survival than those with S100A2/A4-negative tumors (n = 6) (12.5 vs. 20.5 months; P = 0.04). Conclusion: Biomarker assessment from EUS guided biopsy specimens is feasible and successful in 90% of cases. The presence of S100A2 and S100A4 expression predicts both survival and response to pancreatectomy in patients with pancreatic cancer. These findings demonstrate a “proof-of-concept”, that pre-operative EUS guided biopsy could inform clinical decision-making, particularly with regard to selection for operative resection of PDAC. S HEW, W YU, S ROBSON, G STARKEY, A TESTRO, M FINK, P GOW Liver Transplant Unit, Austin Health, Heidelberg, Victoria, Australia 3084 Introduction: Umbilical hernia is a common complication

MCE公司 in patients with end stage liver disease. The risks and outcomes associated with surgical repair in this population have not been clearly defined. The aim of this study was to examine the outcome of umbilical hernia repair in patients with cirrhosis and compare this with a control group of non-cirrhotic patients. Methods: Prospective data was collected using a surgical database recording the outcome of umbilical hernia repairs performed between 2004 and 2013 at the Austin Hospital, Heidelberg, Australia. Data collected included age, sex, severity of liver disease, complications and mortality. Outcomes were compared between patients with and without cirrhosis. Results: 79 patients with cirrhosis (76% male, median age 57 years) and 119 controls (62% male, median age 52 years) were included. Of the patients with cirrhosis, 9% were Child-Pugh A, 61% were Child-Pugh B and 30% were Child-Pugh C. The median Model for End-Stage Liver Disease score was 13 (range 3 to 28). Emergency repairs for incarcerated or perforated hernias comprised 18% in patients with cirrhosis and 6% in controls. The median length of stay for patients with cirrhosis was 3 days (range 1–30) compared with 1 day (range 1–19) in controls (p < 0.01).