74, 1.38-2.19, p<0.001) and liver stiffness > 6 kPa (OR 1.80, 1.52-2.14, p<0.001). A liver biopsy was available in 423 cases. Steatosis was < 10% in 205 cases (48.5%), between 11 and 33% in 85 cases (20.1%), between 34 and 66% in 71 cases (16.8%), and > 66% in 62 cases (14.7%). CAP was correlated with steatosis (r=0, 456, p<0.0001) but not with fibrosis (r=0, 095, p=0, 01). For the diagnosis of steatosis > 10%, steatosis > 33%, and steatosis > 66%, AUROCs of CAP were 0.79 (95%CI 0.74-0.84, p<0.0001), 0.84 (95%CI 0.80-0.88, p<0.0001), 0.84 (95%CI 0.80-0.88, p<0.0001), respectively. Conclusion. CAP is a promising tool for the noninvasive quantification of steatosis. Moreover, its strong

association DAPT purchase with alcohol use and metabolic syndrome could be useful for the follow-up of NAFLD and alcoholic patients in clinical routine and trials. Disclosures: Victor de Ledinghen – Advisory Committees or Review Panels: Merck, Janssen, Gilead, Echosens, Boehringer Ingelheim, Abbvie; Grant/Research Support: Roche, Gilead, Janssen; Speaking and Teaching:

Roche, Echosens Juliette Foucher – Board Membership: roche; Speaking and Teaching: BMS, MSD, Gilead The following people have nothing to disclose: Julien Vergniol, Faiza Chermak, Wassil Merrouche, Maylis Capdepont, Brigitte Le Bail Background and Aims: With the rising B-Raf cancer incidence of non-alcoholic fatty liver disease (NAFLD) with potentially progressive steatohepatitis (NASH) non-invasive tools for risk stratification and follow-up are urgently needed. We therefore used high field magnetic-resonance spectroscopy (MRS) as non-invasive tool to assess NASH and fibrosis profiles and obtain novel mechanistic and pathogenetic insights into alterations of hepatic metabolism in NAFLD. Methods: MRS and liver biopsy were performed back-to-back on the 上海皓元医药股份有限公司 same day in suspected NAFLD/NASH patients and data were correlated with histology (Kleiner/SAF). Hepatocellular lipid content (HCL) was measured by 3.0-T 1H-MRS and 7.0-T 31P-MRS was applied to determine

phosphomonoester (PME), phosphodiester (PDE), phosphocreatine (PCr), NADPH, inorganic phosphate (Pi), a-, p- and y-ATP as well as total phosphate (TP). Results 24 patients (10 female, 14 male) were included. Median age was 51 years (24-70). Histological diagnosis was simple steatosis (SS; n=5) and NASH (n=19; 4 w/ cirrhosis). No difference in BMI or waist-to-hip ratio was observed between both groups. Steatosis assessed by 1H-MRS correlated well with histological data which was improved using a non-linear logarithmic equation [Y=-2.282+25.021*LOG(FAT)] resulting in a better r2 value (r2=0.727; p<0.001) leading to a more accurate measurement of lower grades of HCL. All NASH patients showed significantly higher values of steatosis in histology and MRS compared to SS (r=0.69, p<0.001). A distinction between SS and NASH was feasible by MRS (Cut-off: 0.41; AUROC 0.795, PPV 0.93, NPV 0.5).