, 2005). Almost one-third of the remaining ≥fivefold induced loci represent target genes of ECF σ factors, predominantly σM, with its own autoregulated operon sigM-yhdLK being approximately eightfold induced (Table 3 and Fig. 1a). As a result of a previously described regulatory overlap between different ECF σ factors of B. subtilis (Qiu & Helmann, 2001; Mascher et al., 2007), expression of some genes, such as bcrC and

ywaC, can be regulated by more than one ECF σ factor. But the autoregulated loci of the remaining six ECF σ factors of B. subtilis were not significantly induced (≤threefold), indicating that the ECF response to rhamnolipids is mediated mainly by σM. This ECF σ factor is activated by cell LEE011 supplier wall antibiotics like vancomycin, bacitracin, and phosphomycin, but also under acid, salt, and heat stress conditions (Cao et al., 2002a, b; Mascher et al., 2003; Thackray & Moir, 2003). Other genes significantly

CAL 101 induced by rhamnolipids cannot be assigned to known cell envelope stress regulons. They often encode proteins of unknown function or proteins presumably involved in metabolic and redox processes (e.g. gabD encoding a succinate-semialdehyde dehydrogenase or trxA encoding thioredoxin). We verified the main findings of our DNA microarray analysis, in particular the activation of the TCS LiaRS and CssRS as well as σM, independently by real-time RT-PCR and basically obtained the same results, albeit with an overall higher induction ratio (Fig. 1b). Such discrepancy was observed in numerous studies before and is attributed to the overall lower dynamic range of DNA microarrays compared with other methods such as real-time RT-PCR (Conway & Schoolnik, 2003; Pappas et al., 2004). Treatment with rhamnolipids also led to decreased expression of a certain set of genes (Fig. 1a and Table 3). Among the ≥fivefold repressed loci are genes encoding proteins involved in purine and pyrimidine biosynthesis (pyr and pur operon), phosphate transport (pstSCABABB) and sugar metabolism (rbsRKDACB, xylAB) (Table 2).

Differential expression of the pyr operon in response to cell envelope stress has Lumacaftor order been observed previously for B. licheniformis (Wecke et al., 2006). With almost 20-fold repression, the most strongly downregulated gene is des, which encodes a fatty acid desaturase (Aguilar et al., 1998). Expression of des is controlled by the TCS DesRK and induced by cold shock. The desaturase is important for maintaining membrane fluidity at low temperature by introducing double bonds in phospholipids (Aguilar et al., 2001), indicating that rhamnolipid treatment at sublethal concentrations could interfere with membrane fluidity. Our DNA microarray analysis clearly indicates that rhamnolipids induce both the cell envelope and the secretion stress response. To further validate this novel induction pattern, we performed hierarchical clustering analysis using transcriptome data of B.