05. A total of 1020 unique probe identifiers were significantly

differentially expressed following exposure to MSC, and of these, 979 were deemed “present” (i.e., signal intensity significantly above background). Following exposure to TSC, 557 probes were significantly differentially expressed and 527 were deemed “present”. Of these, 356 were common to both MSC and TSC exposures. The number of significantly up- VEGFR inhibitor and down-regulated genes at each time point and concentration is shown in Table 1. Overall, there was an increase in the number of differentially expressed genes with increasing concentration of condensate, and there were more genes changing after the four hour recovery. At the highest concentration for both time points, cells exposed to MSC had a greater number of changing see more genes (both up and down-regulated) as compared to cells exposed to TSC. Gene expression was most altered for cells exposed to the highest concentration of MSC at the 6 + 4 h time point. Whether separated by dose (data not shown) or considered all together (Fig. 1), Venn diagrams show considerable overlap in the genes that are significantly expressed at each time point following MSC or TSC exposure. Hierarchal clustering using all genes that were statistically significant (i.e., induced

by either TSC or MSC) revealed that the controls and the marijuana high concentration (both time points) clustered independently from the rest of the samples. The remaining samples clustered first by concentration (high, medium, low), then by condensate type (MSC or TSC), with the last branching resulting from time (Fig. 2a). When cells exposed to TSC and MSC were analyzed separately, samples clustered first by concentration and then

by time point, suggesting that concentration has the largest overall effect on gene expression. For MSC, the high concentration L-NAME HCl samples were on the first main branch, followed by control, low and medium concentrations. The results indicate that the expression profiles of the high concentration MSC exposed cells are quite distinct (Fig. 2b.). For TSC, the controls branched separately from all the treatment groups (Fig. 2c.). The top 10 genes with the largest overall fold changes are listed in Table 2. All of the top 10 genes were significantly up-regulated with the exception of low density lipoprotein receptor (Ldlr), which was down-regulated in MSC exposed cells. Of the top 10 changing genes, five genes (Ifrd1, Tiparp, Maff, Atf3, Ptgs2) were common to both MSC and TSC. The GO terms (Biological Process) associated with these common genes included multicellular organismal development, vasculogenesis, regulation of transcription, and regulation of inflammatory response. Ingenuity Pathway Analysis (IPA) was used to define the pathways that were significantly altered following exposure to MSC or TSC. Fig.