0001). Furthermore, these patients with DSAb and AMR had significantly lower death censored allograft survival than both patients without DSAb and patients with DSAb but no AMR.5 The number,

cumulative strength and class of DSAb were not different between patients with DSAb and AMR and patients with DSAb but no AMR. This study supports the prediction that our patient was at an elevated risk of AMR and therefore lower death censored allograft survival. The complexity, however, in a broadly sensitized patient such as ours, is in deciding which DSAb and at what MFI is the risk of proceeding acceptable given that they are BVD-523 datasheet unlikely to ever get a transplant offer that avoids all DSAb. Clearly not all anti-HLA antibodies are equal with regard to the ability to fix complement and not all DSAb-positive patients progress to AMR. While missing donor HLA typing was an issue in interpreting the Luminex results in the case presented, there are also some deficiencies with antigen-coated bead technology which can influence interpretation. Among these is the finding that there is considerable variability in the density

of antigen representation on the SAB in the commercially available assays. A previous report related the antigen density on the SAB to their relative sensitivity in detecting alloantibodies with HLA density ranging from 10.1 molecules of equivalent soluble fluorochrome see more (MESF) on the HLA-A69 SAB to 333.6 MESF on the HLA-A31 SAB.6 The antigen density on class II SAB beads also varied considerably between samples lot to lot. Clearly such differences in antigen density will affect the read-out in terms of perceived antibody strength, most commonly reported in terms of MFI, which may lead to inconsistent correlations with CDC crossmatch results and ultimately this may influence decision making. Single antigen beads are limited to the number of beads in the kit, therefore HLA antigens are not all represented, (-)-p-Bromotetramisole Oxalate uncommon HLA

are often absent. Antibodies to a donor with an uncommon HLA may be missed. Additionally, technical issues whereby manufacturing processes lead to denatured HLA on the beads exposing cryptic epitopes and false reactivity that is not truly HLA-specific can corrupt results. Some patients have a high degree of non-specific reactivity against solid phase assays, making accurate identification of HLA alloantibodies difficult. In concluding, this case highlights immunological limitations and dilemmas in our current transplant decision-making processes. Incomplete prospective deceased donor HLA typing and the limitations in antibody detection remain major current issues. Despite these limitations the increasing sophistication in antibody detection techniques and HLA typing has added to the clinician’s ability to stratify the immunological risk associated with each donor recipient transplant combination.