In support of the presumed role of SbnA and SbnB in L-Dap synthesis, Thomas and colleagues [18] earlier

proposed that the enzymes VioB (SbnA homologue) and VioK (SbnB homologue) were involved in production of L-Dap for viomycin synthesis. Therefore, it appears that homologues of sbnA and sbnB are widely distributed across biosynthetic gene clusters, whose enzymes synthesize molecules for which L-Dap is featured as a structural component. Table 2 List of SbnA homologs Organism Similar Proteina PDB ID Identity (%) Similarity E-Value Arabidopsis thaliana Cysteine Angiogenesis inhibitor synthase 1z7w 33 0.500 0 Homo sapiens Cystathionine beta-synthase 1jbq 30 0.498 0 Schizosaccharomyces pombe selleck inhibitor Serine racemase 1v71 17 0.202 0 Escherichia coli Biosynthetic threonine deaminase 1tdj 18 0.255 0 Homo sapiens L-serine dehydratase 1p5j 20 0.249 0 Mycobacterium tuberculosis Threonine synthase 2d1f 19 0.279 0 Pyrococcus furiosus Tryptophan synthase beta chain 1 1v8z 22 0.231 0 Pyrococcus horikoshii 1-aminocyclopropane-1-carboxylate deaminase 1j0a 19 0.123 0 aOnly top hit, using HHPred, for each class of enzyme is shown Table 3 List of SbnB homologs Organism Homologous Proteina PDB ID Identity (%) Similarity E-Value Archaeoglobus fulgidus Alanine dehydrogenase 1omo 32 0.543 0 Homo sapiens MU-crystallin homolog 2i99 24 0.381 0 Pseudomonas putida Ornithine cyclodeaminase 1x7d 21 0.352 0 Thermoplasma volcanium Glutamyl-tRNA reductase 3oj0 15 0.312 3e-19 Geobacillus

kaustophilus Shikimate 5-dehydrogenase 2egg 13 0.113 1.4e-9 aOnly top hit, using HHPred, for each AZD5363 in vivo class of enzyme is shown Table 4 List of bacteria, not including S. aureus, containing transcriptionally-linked sbnA/sbnB homologs Organism Homologous Gene ID (SbnA/SbnB) Similarity

(% SbnA/%SbnB) E-Value (SbnA/SbnB) Predicted gene cluster product Staphylococcus pseudintermedius HK10-03 spint_0334/spint_0335 90/91 2e-149/6e-161 Staphyloferrin B Cupriavidus metallidurans Histamine H2 receptor CH34 rmet_1117/rmet_1116 75/71 1e-102/2e-97 Staphyloferrin B Ralstonia solanacearum GMT1000 cysK2/rsp0418 76/79 8e-100/2e-118 Staphyloferrin B Shewanella denitrificans OS217 sden_0590/sden_0589 73/75 6e-100/2e-109 Staphyloferrin B Methylobacterium nodulans ORS 2060 mnod_6948/mnod_6949 71/72 9e-91/2e-104 Staphyloferrin B Acinetobacter sp. DR1 aole_07120/aole_07115 76/74 4e-84/6e-104 Staphyloferrin B?** Clostridium cellulovorans 743B clocel_3151/clocel_3150 64/55 4e-65/7e-39 Unknown Streptomyces griseus subsp. Griseus NBRC 13350 sgr_2592/sgr_2591 57/52 1e-56/9e-34 Unknown NRPS product Pantoea agglomerans ddaA/ddaB 56/53 5e-57/3e-32 Dapdiamide antibiotic Bacillus thuringiensis serovar kurstaki YBT-1520 zwa5A/zwa5B 63/55 5e-72/3e-48 Zwittermicin A antibiotic Streptomyces vinaceus vioB/vioK 52/47 1e-49/3e-31 Viomycin antibiotic Acidobacterium capsulatum ATCC 51196 acp_1153* 61/44 5e-73/1e-26 Unknown polyketide-NRPS product Pseudomonas syringae pv. tomato DC3000 pspto_2960* 59/49 4e-64/4e-34 Unknown Paenibacillus sp.