Several studies found that protease activity of fecal or mucosa was increased in IBS patients, meanwhile the increased protease activity could activate protease activated receptor 2 to induce nociceptive neurons high excitability, which is more concerned an important mechanism in recent years leading to visceral hypersensitivity. we will observe the effect of protease inhibitors on visceral sensitivity and spinal c-fos expression induced by acute stress. Methods: The acute stress was induced by wrapping the fore shoulders, upper forelimbs and thoracic trunk of Sprague-Dawley rats for 2 h. Either camostat mesilate (CM) (30, 100 or 300 mg/kg, respectively) or saline was intragastrically administrated
to the rats 30 min before the acute restraint stress. Visceral perception was quantified as visceral motor response with an electromyography in rats. The spinal c-fos expression was measured by immunohistochemistry. Napabucasin Results: CM inhibits the visceral sensitization in a dose-dependant manner
elicited by rectal distension. When the dose of CM was 30 mg/kg, the area under the curve in the stress + CM group with 1.0 ml and ZD1839 molecular weight 1.2 ml of distension volume was decreased compared with that of the stress + vehicle group. When the CM dose increased to 100 mg/kg or 300 mg/kg, CM resulted in a significant effect. The area under the curve in the stress + CM group with 0.8 ml, 1.0 ml and 1.2 ml of distension volume was decreased compared with that of the stress + vehicle group (P < 0.01). However, these doses did not completely prevent the area under the curve elicited by rectal distension in the stress rats. Acute stress increased fos protein expression in the spinal dorsal horn, after administration the protease
inhibitors, the fos protein positive cells decreased. Fos protein mainly expressed in the superal spinal cord dorsal horn in immunohistochemistry. Conclusion: The protease inhibitor inhibits the visceral hypersensitivity and spinal C-fos expression induced by the acute stress. Key Word(s): 1. visceral sensitivity; 2. c-fos; 3. acute stress; 4. protease inhibitor; Presenting Author: JING YANG Additional Authors: YUNSHENG YANG, JING WEN, BIN YAN, ZHONGSHENG LU Corresponding selleck kinase inhibitor Author: YUNSHENG YANG Affiliations: Department of Gastroenterology and Hepatology, Chinese PLA General Hospital; Department of Gastroenterology and Hepatology, Department of Gastroenterology and Hepatology, Chinese PLA General Hospital Objective: To investigate the clinical manifestations, endoscopic and pathologic characteristics of primary small intestinal lymphoma. Methods: The clinical, pathologic and endoscopic data of 40 cases of primary small intestinal lymphoma in our hospital were retrospectively analyzed. Results: Among 40 cases, the most common symptoms were abdominal pain, loss of weight, fever, abdominal mass, blood in stools, and change in bowel habits.